This Python-controlled fraction collector integrates programmable vial positioning with real-time volumetric monitoring, enabling accurate, flow-independent fraction collection. We validated performance across a range of flow rates (0.1–6 mL min−1) and viscosities (0.45–500 cP), demonstrating consistent droplet mass and high reproducibility. The system also delivers high separation fidelity, with sharp fluid transitions and negligible carryover between fractions under both aqueous and organic conditions. The system is highly robust, and all wetted parts are fluoropolymer-based to ensure compatibility with a broad range of organic solvents and reagents. Repoducibility and separation quality studies: - https://pubs.rsc.org/dd/article/5/5/2067/1240065/Python-controlled-solvent-resistant-fraction.
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